Monday, April 27, 2020
An Essay Or a Report? An Urine Sample on a Transmission Electron Microscope
An Essay Or a Report? An Urine Sample on a Transmission Electron MicroscopeThe way you approach an essay or a report depends on what microscope would you use to examine unstained urine samples for contamination? Most of us would naturally choose the microscope that measures and looks at visible light. Most often, this is the most easily accessible microscope in our laboratories and the one that most of us are familiar with.But, perhaps it might surprise you to learn that it is actually a type of microscope that has no visible light and is used instead to look for organisms and compounds, such as DNA, which has the potential to be contaminated by fungi or viruses. In fact, it is the same principle as when you use a UV microscope in order to see minute fungi under a black light.This microscope is called a transmission electron microscope. It is used primarily to look for bacteria, which can grow in a variety of environments including soil, the air, or in water. But, before they are abl e to reproduce in the environment, bacteria need to colonize or be present, so they have a protective layer. This protective layer comes in a variety of forms including an outer film which protects the bacteria, a core shell which can be broken by a UV radiation, or even mucus.Mucus protects the bacteria from an assortment of fungi including the common Black spot (Pseudomonas). The bacteria however, are not just there to protect themselves, they also aid the fungi in their survival by producing enzymes that help them digest the cellulose in plants and animals and produce carbohydrates.The bacteria and fungi live together, but because they are so close to each other they usually have very little contact with each other. Because of this, a UV light is commonly used in order to illuminate them and reveal any potential problems. Once you've gotten a handle on what specific bacteria and fungi you are looking for, you can go on to take your microscope to the next step and use your microsc ope to look for DNA.A transmission electron microscope allows you to look at DNA and other forms of genetic material. As soon as they are seen, it is extremely important to keep them from getting mixed up in the mix of other DNA, as it could cause the DNA to mutate and cause sickness. The more pieces of DNA you have to work with, the more difficult it is to identify different parts of DNA and thus the more time you will need to spend examining a urine sample.One way to help you is to take a few different samples and then keep them separate and analyze them on different days. Once you've identified what you're looking for, you should try to determine the best method of storage for your specimens. If you do it right, you can keep your specimens in the freezer and allow them to freeze, then just reheat the specimens and use them in an assignment.
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